KMID : 0525720160210020094
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Journal of Chitin and Chitosan 2016 Volume.21 No. 2 p.94 ~ p.99
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Production of Minor Ginsenoside F2 from Ginseng Extracted Saponins by Viscozyme¢ç L
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Kim Do-Yeon
Park Chang-Su Kim Yeong-Su
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Abstract
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The commercial enzyme Viscozyme ¢ç L was used for bioconversion of major PPD type ginsenosides to ginsenoside F2. The optimal conditions for production of ginsenoside F2 from PPD type ginsenosides by Viscozyme ¢ç L were pH 6.0, 50¡ÆC, and 60 U/mL enzyme. Interestingly, the enzyme hydrolyzed only outer ¥â-(1,2)-D-glucose linkage at C-3, C-6, and C-20 position in ginsenosides. Under optimum conditions, the enzyme converted ginsenoside Rb1, Rb2, Rc, and Rd in ginseng extract to ginsenoside F2 with a molar yield of over 95% and productivity of 0.7 g/L/h within 8 h.
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KEYWORD
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Ginseng, Ginsenoside, Ginsenoside F2, Viscozyme¢ç L, Bioconversion
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